An oncolytic/immunogenic complementary-adenoviral vector system

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 35/76 (2006.01) A61K 48/00 (2006.01) C07K 14/47 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2280237

This invention encompasses a composition for killing target cells, such as tumor cells. The composition comprises a first and a second adenoviral vector that have complementary function and are mutually dependent on each other for replication in a target cell. One of said adenoviral vectors has a target cell- activated promoter or a functional deletion that controls and limits propagation of the adenoviral vectors in the target cells which directly or indirectly kills the target cells. One of the adenoviral vectors comprises a gene encoding a protein which is expressed in the target cells and can induce anticancer immune responses. The target cells may be hepatoma, breast cancer, melanoma, colon cancer, or prostate cancer cells, for example. The vectors of this invention may also be utilized to treat other diseases such as restenosis, in which case the target cell may be a vascular smooth muscle cell, for example.

L'invention concerne une composition pour tuer des celles cibles telles que des cellules tumorales. Ladite composition comprend des premier et deuxième vecteurs adénoviraux ayant une fonction complémentaire et dépendant l'un de l'autre pour la réplication dans une cellule cible. Un desdits vecteurs adénoviraux comprend un promoteur activé par la cellule cible ou une délétion fonctionnelle qui commande et limite la propagation des vecteurs adénoviraux dans les cellules cibles, ce qui tue directement ou indirectement les cellules cibles. Un des vecteurs adénoviraux comprend un gène codant une protéine qui est exprimée dans les cellules cibles et peut induire des réponse immunitaires anticancéreuses. Les cellules cibles peuvent être, entre autres, des cellules d'hépatocarcinome, de cancer du sein, de cancer du colon, de mélanome ou de cancer de la prostate. Les vecteurs de l'invention peuvent également être utilisés pour le traitement d'autres maladies telles que la resténose, auquel cas la cellule cible peut être, entre autres, un cellule de muscle lisse de la paroi vasculaire.

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