An unusual retrotransposon from the yeast candida albicans

Details An unusual retrotransposon from the yeast candida albicans An unusual retrotransposon from the yeast candida albicans

C - Chemistry, Metallurgy

12

N

C12N 15/81 (2006.01) C12N 9/12 (2006.01) C12N 15/31 (2006.01) C12N 15/90 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2249046

The invention relates to a novel retrotransposon isolated from the yeast Candida albicans. The new retrotransposon, designated pCal, belongs to the Ty1/Copia group. The free, linear, double stranded DNA form of the element is highly expressed such that it can seen as a distinct band when uncut genomic C.albicans DNA is analysed on a agrose gel. The retrotransposon has a copy number of between 40 and 150 DNA copies of itself per genome and may be used in a method of introducing DNA into the genome of a cell. It may also be used in a gene disruption and/or gene discovery system.

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