An unusual retrotransposon from the yeast candida albicans

C - Chemistry – Metallurgy – 12 – N

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C12N 15/87 (2006.01)

Patent

CA 2348737

TCa2 is a Tyl/copia retrotransposon from the pathogenic yeast Candida albicans. In contrast to other retrotransposons it can appear as an abundant, extrachromosomal double-stranded DNA molecule, called pCal. The invention relates to the isolation and characterisation of TCa2 and pCal together with its uses for inducing random mutagenesis in a genome, as a component of a transposable element and of an expression vector.

TCa2 est un rétrotransposon Tyl/copia provenant d'un Candida albicans pathogène. Contrairement aux autres rétrotransposons, le rétrotransposon de l'invention peut se présenter sous la forme d'une molécule d'ADN double brin extrachromosomique abondante, appelée pCal. L'invention concerne l'isolement et la caractérisation de TCa2 et de pCal, ainsi que leur utilisation en vue d'induire la mutagenèse aléatoire dans un génome, en tant que composant d'un élément transposable et d'un vecteur d'expression.

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