Analgesic peptides from venom of grammostola spatulata and...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 14/435 (2006.01) A61K 38/17 (2006.01) C07K 16/18 (2006.01) G01N 33/68 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2319038

The present invention provides novel methods of treating pain comprising administering to a mammal in need of such treatment an effective analgesic amount of a peptide having the amino acid sequence Tyr-Cys-Gln-Lys-Trp-Leu-Trp- Thr-Cys-Asp-Ser-Glu-Arg-Lys-Cys-Cys-Glu-Asp-Met-Val-Cys-Arg-Leu-Trp-Cys-Lys- Lys-Arg -Leu-NH2 (SEQ ID NO:1) or Tyr-Cys-Gln-Lys-Trp-Met-Trp-Thr-Cys-Asp-Glu- Glu-Arg-Lys-Cys-Cys-Glu-Gly-Leu-Val-Cys-Arg-Leu -Trp-Cys-Lys-Lys-Lys-Ile-Glu- Trp (SEQ ID NO:2). The invention further provides a purified peptide having the amino acid sequence of SEQ ID NO:1. The peptides of SEQ ID NO:1 and SEQ ID NO:2 can also be used in methods for identifying compounds having analgesia- inducing activity.

L'invention porte sur de nouvelles méthodes de traitement de la douleur consistant à administrer à un mammifère le nécessitant une dose à effet analgésique d'un peptide présentant soit la séquence d'acides aminés: Tyr-Cys-Gln-Lys-Trp-Leu-Trp-Thr-Cys-Asp-Ser-Glu-Arg-Lys-Cys-Cys-Glu-Asp-Met-Val-Cys-Arg-Leu-Trp-Cys-Lys-Lys-Arg-Leu-NH2 (SEQ ID NO:1), soit la séquence d'acides aminés: Tyr-Cys-Gln-Lys-Trp-Met-Trp-Thr-Cys-Asp-Glu-Glu-Arg-Lys-Cys-Cys-Glu-Gly-Leu-Val-Cys-Arg-Leu-Trp-Cys-Lys-Lys-Lys-Ile-Glu-Trp (SEQ ID NO:2. L'invention porte également sur un peptide purifié présentant la séquence d'acides aminés SEQ ID NO:1. Les peptides des séquences SEQ ID NO:1 et SEQ ID NO:2 peuvent de plus être utilisés dans des méthodes d'identification de composés analgésiogènes.

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