Analyses of biological fluids

Details Analyses of biological fluids Analyses of biological fluids

G - Physics

01

N

167/37

G01N 33/92 (2006.01) G01N 33/54 (1980.01)

Patent

CA 1186220

Abstract Analyses of Biological Fluids An immunologic assay is taught for determining the presence of one or more phospholipids in a biological fluid. The method includes adding an ethanolic solution of diacylphosphatidylcholine or alkyltrimethylammonium halide and cholesterol to the biological fluid forming a macromolecular aggregate complex suspension. To the macromolecular aggregate complex solution is then added either an additional quantity of the biological fluid or a buffer reagent. The product of the reaction is then added to antibody molecules to the phospholipid and examined to determine the presence of the phospho- lipids. The method is particularly useful in determining the presence of phosphatidylglycerol in a sample of amniotic fluid.

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