Analysis chip, analysis method and method for stirring solution

G - Physics – 01 – N

Patent

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Details

G01N 35/02 (2006.01) C12M 1/00 (2006.01) C12N 15/09 (2006.01) G01N 37/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2755709

[Object] The invention provides an analysis chip by which the efficiency of reaction with a selective binding substance is promoted by using particles having a large size to promote the efficiency of stirring of the test substance solution without damaging the portion of the carrier on which the selective binding substance is immobilized, by the moving particles when stirring the test substance solution with the particles. [Means of Solution] An analysis chip including a carrier having a surface on which a selective binding substance(s) is(are) immobilized; a vessel holding a solution containing a test substance(s) which react(s) with the selective binding substance(s); and particles for stirring the solution, the particles being sealed within a space formed between the carrier and the vessel; wherein a separator which allows passage therethrough of the solution containing the test substance(s) but does not allow passage therethrough of the particles is arranged in the space so as to separate the surface of the carrier on which the selective binding substance(s) is(are) immobilized and the particles; a method of analysis using the analysis chip; and a method of stirring a solution using the analysis chip.

La puce d'analyse selon l'invention permet, dans un procédé d'agitation d'une solution d'une substance d'essai avec des microparticules, d'augmenter l'efficacité d'agitation de la solution de la substance d'essai en utilisant des microparticules de grande taille, sans endommager sous l'effet de la migration des microparticules une partie de support sur lequel une substance se liant sélectivement est immobilisée, pour accroître ainsi l'efficacité de réaction avec la substance se liant sélectivement. La puce d'analyse selon l'invention comprend : un support sur la surface duquel une substance se liant sélectivement est immobilisée ; un récipient qui renferme une solution contenant une substance d'essai capable de réagir avec la substance se liant sélectivement ; et des microparticules pour agiter la solution qui sont confinées dans un espace formé par le support et le récipient, ladite puce d'analyse étant caractérisée en ce qu'une cloison, qui permet la perméation de la solution contenant la substance d'essai mais pas celle des microparticules, est insérée dans l'espace de façon à séparer les microparticules de la face du support sur laquelle la substance se liant sélectivement est immobilisée. Un procédé d'analyse utilisant la puce d'analyse selon l'invention et un procédé pour agiter une solution sont également décrits.

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