C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
150/3.3, 150/15.
C12Q 1/60 (2006.01) C12N 9/10 (2006.01)
Patent
CA 1182727
Abstract of the Disclosure Methods, compositions and elements for the analysis of total cholesterol in aqueous fluids con- taining cholesterol and cholesterol esters are dis- closed. Cholesterol esters in the fluid samples are first converted to cholesterol by: (a) contacting a sample of the fluid with sufficient amounts of lysolecithin and an enzyme exhibiting lecithin:cholesterol acyl transferase (LCAT) activity to convert substantially all the choles- terol esters to cholesterol in the sample, and (b) removing cholesterol from the aqueous fluid other than by conversion to cholesterol esters. The total cholesterol is quantitatively determined by measuring the amount of the removed cholesterol. For example, the amount is measured gravimetrically or by converting the cholesterol to a detectable product.
413204
Eastman Kodak Company
Gowling Lafleur Henderson Llp
LandOfFree
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