Analysis method and system therefor

G - Physics – 01 – N

Patent

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Details

G01N 33/48 (2006.01) G01N 21/25 (2006.01) G01N 21/31 (2006.01)

Patent

CA 2468513

The invention concerns a method for quantitative hemoglobin determination in undiluted, unhemolyzed whole blood comprising the steps of: providing a disposable, capillary, which has an optical path length of less than 1 mm; filling said cuvette with a sample of unaltered whole blood; performing a first absorption measurement at a wavelength in the range 490-520 nm directly on the sample in the cuvette, and further conducting a second absorption measurement, and processing results of the first and second absorption measurements to determine the concentration of hemoglobin in the sample, wherein the step of processing comprises compensating for scattering in the sample, said compensating being dependent on the result of the second absorption measurement. A system for implementing the method is also disclosed.

La présente invention concerne un procédé de mesure quantitative de l'hémoglobine dans le sang entier non hémolysé non dilué qui comprend les étapes suivantes : on obtient un capillaire jetable d'une longueur de trajet optique inférieure à 1 mm ; on remplit la cuvette avec un échantillon de sang entier non modifié ; on effectue une première mesure d'absorption à une longueur d'onde comprise entre 490 et 520 nm directement sur l'échantillon dans la cuvette ; on procède à une seconde mesure d'absorption ; et on traite les résultats de la première et de la seconde mesure d'absorption afin de déterminer la concentration en hémoglobine de l'échantillon, l'étape de traitement consistant à compenser la diffusion dans l'échantillon, ladite compensation dépendant du résultat de la seconde mesure d'absorption. L'invention se rapporte également à un système destiné à la mise en oeuvre du procédé précité.

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