Analysis method using a non-hygroscopic hemolysing agent and...

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G01N 21/31 (2006.01) G01N 21/03 (2006.01) G01N 21/27 (2006.01) G01N 33/50 (2006.01) G01N 33/72 (2006.01)

Patent

CA 2414316

The present invention concerns a method for hemoglobin determination which comprises the steps of introducing a sample of undiluted whole blood by capillary action into a disposable microcuvette having at least one cavity for receiving the sample. The cavity includes a dry essentially non-hygroscopic hemolysing agent, which is dissolved by the blood, hemolyses the red blood cells and releases the haemoglobin contained in the blood cells. A first absorption measurement at a wavelength range 490-520 nm is then performed directly on the sample in the microcuvette, and a second absorption measurement is performed to compensate for background interference.

La présente invention concerne une méthode de détermination d'hémoglobine, consistant à introduire un échantillon de sang entier non dilué par cation capillaire dans une microcuvette jetable possédant au moins une cavité destinée à recevoir l'échantillon. Ladite cavité comprend un agent d'hémolyse sec essentiellement non hygroscopique qui est dissout dans le sang, produit l'hémolyse des globules rouges et libère l'hémoglobine contenue dans les cellules sanguines. Une première mesure d'absorption à une longueur d'onde de 490 à 520 nm est alors effectuée directement sur l'échantillon dans la microcuvette, puis une seconde mesure d'absorption est effectuée pour compenser les interférences de fond.

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