Analysis of carbohydrates

Details Analysis of carbohydrates Analysis of carbohydrates

G - Physics

01

N

G01N 33/52 (2006.01) G01N 21/64 (2006.01) G01N 27/447 (2006.01) G01N 33/533 (2006.01)

Patent

CA 2267337

A method of separating or distinguishing carbohydrate substances, by the use of a fluorescent labelling reagent which has a positive charge when bound to a carbohydrate, involves separating or distinguishing labelled carbohydrate substances, e.g. by performing electrophoresis to cause differential migration of different labelled carbohydrate substances, or by isoelectric focusing in a pH gradient. Preferably the fluorescent labelling reagent is a cyanine dye.

Procédé pour séparer ou distinguer des matières glucidiques dans lequel on utilise un réactif de marquage fluorescent, qui présente une charge positive lorsqu'il est lié à un glucide. Le procédé consiste à séparer ou distinguer les matières glucidiques marquées p. ex. par électrophorèse de manière à entraîner une migration différentielle des diverses matières glucidiques marquées, ou par focalisation isoélectrique sur gradient pH. Le réactif de marquage est de préférence un colorant cyanine.

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