Analysis of carbohydrates in biological fluids by high...

G - Physics – 01 – N

Patent

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Details

G01N 33/50 (2006.01) G01N 30/02 (2006.01) G01N 33/66 (2006.01) G01N 30/06 (2006.01) G01N 30/88 (2006.01)

Patent

CA 2238856

The present invention is directed to a method of detecting reducing carbohydrates in a complex biological sample. The sample is filtered, contacted with an anion exchange resin, derivatized with a 3-alkyl-5- pyrazolinone derivative, purified by liquid-liquid extraction and separated and detected by HPLC. The method can be used to monitor either various disease states or response to oligosaccharide therapy.

L'invention concerne un procédé servant à détecter des glucides réducteurs dans un spécimen biologique complexe. Ce spécimen est filtré, mis en contact avec une résine échangeuse d'anions, dérivé au moyen d'un dérivé de 3-alkyle-5-pyrazolinone, purifié par extraction liquide-liquide, séparé et détecté par chromatographie liquide à performance élevée (HPLC). Ce procédé peut être utilisé afin de contrôler soit différents états de maladies, soit une réaction à une thérapie d'oligosaccharides.

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