Analysis of circulating tumor cells, fragments, and debris

Details Analysis of circulating tumor cells, fragments, and debris Analysis of circulating tumor cells, fragments, and debris

C - Chemistry, Metallurgy

12

Q

C12Q 1/04 (2006.01) A01N 1/02 (2006.01) B03C 1/00 (2006.01) C12Q 1/02 (2006.01) C12Q 1/24 (2006.01) G01N 33/50 (2006.01) G01N 33/53 (2006.01) G01N 33/543 (2006.01) G01N 33/569 (2006.01) G01N 33/574 (2006.01)

Patent

CA 2457894

The methods and reagents described in this invention are used to analyze circulating tumor cells, clusters, fragments, and debris. Analysis is performed with a number of platforms, including flow cytometry and the CellSpotter®* fluorescent microscopy imaging system. Analyzing damaged cells has shown to be important. However, there are two sources of damage: in vivo and in vitro. Damage in vivo occurs by apoptosis, necrosis, or immune response. Damage in vitro occurs during sample acquisition, handling, transport, processing, or analysis. It is therefore desirable to confine, reduce, eliminate, or at least qualify in vitro damage to prevent it from interfering in analysis. Described herein are methods to diagnose, monitor, and screen disease based on circulating rare cells, including malignancy as determined by CTC, clusters, fragments, and debris. Also provided are kits for assaying biological specimens using these methods.

L'invention concerne des procédés et des réactifs mis en oeuvre pour analyser des cellules tumorales en circulation, des agrégats, des fragments et des débris associés. L'analyse est effectuée au moyen d'un certain nombre de plates-formes, notamment une cytométrie de flux et le système d'imagerie par microscopie fluorescente CellSpotter®*. L'analyse de cellules endommagées s'est avérée très importante. Cependant, il y a deux sources de dommages: <i>in vivo</i> et <i>in vitro</i>. Des dommages <i>in vivo</i> sont causés par apoptose, nécrose ou réponse immune. Des dommages <i>in vitro</i> apparaissent au cours de l'acquisition, de la manipulation, du transport, du traitement ou de l'analyse d'échantillons. Il est, par conséquent, souhaitable de limiter, de réduire, d'éliminer ou au moins de qualifier des dommages <i>in vitro</i>, de manière à empêcher ceux-ci d'interférer dans l'analyse. L'invention concerne également des procédés de diagnostic, de surveillance et de balayage de maladie fondés sur des cellules rares en circulation, notamment une malignité telle que déterminée par CTC, des agrégats, des fragments et des débris, ainsi que des kits permettant de doser des spécimens biologiques au moyen de ces procédés.

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