Analysis of dna by means of capillary electrophoresis

Details Analysis of dna by means of capillary electrophoresis Analysis of dna by means of capillary electrophoresis

G - Physics

01

N

G01N 27/447 (2006.01)

Patent

CA 2719429

The present invention relates to a method for detecting nucleic acids, wherein a sample to be analyzed for the presence of nucleic acids is separated by capillary electrophoresis. The conditions of sample injection and separation allow for an extremely high sensitivity of the method, which can be applied, e.g. for quality control purposes in the determination or the presence, quantity and/or size of genomic DNA contaminants in samples comprising proteins for therapy or vaccination.

L'invention concerne un procédé de détection d'acides nucléiques, dans lequel un échantillon analysé en vue de détecter la présence d'acides nucléiques est séparé par électrophorèse capillaire. Les conditions d'injection et de séparation de l'échantillon permettent d'obtenir un procédé présentant une sensibilité extrêmement élevée, qui peut être appliqué par exemple à des fins de contrôle de qualité pour déterminer la présence, la quantité et/ou la taille de contaminants d'ADN génomique dans des échantillons comprenant des protéines et destinés à un traitement thérapeutique ou à une vaccination.

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