Analysis of liquid chromatography eluates

G - Physics – 01 – N

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G01N 30/78 (2006.01) G01N 30/64 (2006.01) G01N 30/74 (2006.01)

Patent

CA 2567528

When analysing saccharides by HPAEC, the eluate from the column is typically analysed using a amperometric detector. According to the invention, amperometric detection is coupled with ultraviolet detection, with both methods being applied to the eluate. Thus the invention provides a method for analysing the eluate from a liquid chromatography column, wherein the eluate is analysed by both (a) amperometric detection and (b) ultraviolet detection. The information content derivable from using both sorts of detection advantageously exceeds that derivable from either of the two detection methods alone.

Lors de l'analyse de saccharides par chromatographie d'échange d'anions haute performance (HPAEC), l'éluat issu de la colonne est en général analysé à l'aide d'un détecteur ampérométrique. Selon l'invention, la détection ampérométrique est associée à la détection UV, les deux techniques étant appliquées à l'éluat. L'invention concerne donc un procédé permettant d'analyser l'éluat issu d'une colonne de chromatographie en phase liquide, qui consiste à analyser l'éluat à la fois par (a) détection ampérométrique et (b) détection UV. Les données pouvant être dérivées des deux types de détection dépassent avantageusement celles qui peuvent être obtenues avec seulement l'une des deux techniques.

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