Analysis of mannosamine-containing capsular saccharides

Details Analysis of mannosamine-containing capsular saccharides Analysis of mannosamine-containing capsular saccharides

G - Physics

01

N

G01N 33/50 (2006.01)

Patent

CA 2659027

Analysis of compositions that include saccharides having mannosamine residues, such as the capsular saccharide of N. meningitidis serogroup A, is facilitated by a method comprising the steps of: (i) hydrolysing polysaccharide in the sample, to give a hydrolysate; (ii) subjecting the hydrolysate to liquid chromatography; and (iii) detecting any mannosamine-6-phosphate separated in step (ii).

L'analyse de compositions qui comprennent des saccharides présentant des résidus de mannosamine, tel que le saccharide capsulaire du sérogroupe A de N. meningitidis, est facilitée par un procédé comprenant les étapes consistant: (i) à hydrolyser un polysaccharide dans l'échantillon, afin d'obtenir un hydrolysat; (ii) à soumettre l'hydrolysat à une chromatographie en phase liquide: et (iii) à détecter une mannosamine-6-phosphate séparée dans l'étape (ii).

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