Analysis of mixtures of nucleic acid fragments and of gene...

Details Analysis of mixtures of nucleic acid fragments and of gene... Analysis of mixtures of nucleic acid fragments and of gene...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2480320

The invention relates to a method for analysing nucleic acid fragments, said method comprising the following steps: a) at least one mixture of nucleic acid fragments is prepared, said mixture having at least one recognition site for a restriction endonuclease cutting outside its recognition site, b) at least part of the mixture of nucleic acid fragments from step (a) is incubated with at least one restriction endonuclease having a cutting site outside its recognition site, and c) at least one nucleotide of the cut nucleic acid fragments from step (b) is identified, and optionally other fragment-specific characteristics of the cut nucleic acid fragments from step (b) are identified, said identification steps being simultaneously carried out for a plurality, or all, of the nucleic acid fragments.

L'invention concerne un procédé pour analyser des fragments d'acide nucléique, comprenant les opérations suivantes: a) préparer au moins un mélange de fragments d'acide nucléique comportant au moins un site de reconnaissance pour une endonucléase de restriction coupant en dehors de son site de reconnaissance ; b) faire incuber au moins une partie du mélange des fragments d'acide nucléique obtenu à l'étape (a) avec au moins une endonucléase de restriction dont le site de coupe se trouve en dehors de son site de reconnaissance ; c) identifier une ou plusieurs nucléotides des fragments d'acide nucléique coupés à l'étape (b) et identifier éventuellement d'autres propriétés spécifiques aux fragments des fragments d'acide nucléique coupés à l'étape (b), cette/ces identification(s) se déroulant simultanément pour plusieurs fragments ou pour tous les fragments d'acide nucléique.

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