Analysis of nucleotide polymorphisms at a site

C - Chemistry – Metallurgy – 12 – Q

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C12Q 1/68 (2006.01)

Patent

CA 2361643

The identity of the polymorphic nucleotide in a target sequence having at least two known variants can be easily and efficiently detected by hybridizing at least one primer upstream of the biallelic marker and performing extension reactions using the target DNA with the hybridized primer, where a first reaction is conducted in the absence of a deoxyribonucleoside triphosphate or ribonucleoside triphosphate complementary to the first known variant, and a second reaction is conducted in absence of a deoxyribonucleoside triphosphate or ribonucleoside triphosphate complementary to the second known variant. Determining the lengths of the primers and any extension products from both reactions will indicate which variant or variants are present in a DNA sample.

L'identité du nucléotide polymorphe dans une séquence cible présentant au moins deux variantes connues, peut être facilement et efficacement détectée par hybridation d'au moins un flux montant d'amorce du marqueur biallélique, puis par déclenchement de réactions d'extension à l'aide de l'ADN cible avec l'amorce hybridée, une première réaction ayant lieu en l'absence d'un déoxyribonucléoside triphosphate ou d'un ribonucléoside triphosphate complémentaire de la première variante connue, et une seconde réaction ayant lieu en l'absence d'un déoxyribonucléoside triphosphate ou d'un ribonucléoside triphosphate complémentaire de la seconde variante connue. L'analyse de la longueur des amorces ainsi que de tout produit d'extension provenant des deux réactions, permettra d'indiquer la nature de/des variantes présentes dans l'échantillon d'ADN.

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