Analyte assay using particulate labels

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C12Q 1/00 (2006.01) C12Q 1/70 (2006.01) C40B 30/04 (2006.01) C40B 70/00 (2006.01) G01N 15/14 (2006.01) G01N 21/62 (2006.01) G01N 33/48 (2006.01) G01N 33/483 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01)

Patent

CA 2307280

Method for specific detection of one or more analytes in a sample. The method includes specifically associating any one or more analytes in the sample with a scattered-light detectable particle, illuminating any particle associated with the analytes with light under conditions which produce scattered light from the particle and in which light scattered from one or more particles can be detected by a human eye with less than 500 times magnification and without electronic amplification. The method also includes detecting the light scattered by any such particles under those conditions as a measure of the presence of the analytes.

L'invention concerne un procédé de détection spécifique d'un ou de plusieurs analytes dans un échantillon. Le procédé comporte les étapes consistant à associer de manière spécifique un ou plusieurs analytes présents dans l'échantillon à une particule détectable à lumière diffusée, éclairer une particule associée aux analytes au moyen de lumière dans des conditions qui permettent la production de lumière diffusée provenant de la particule ainsi que la détection de lumière diffusée provenant d'une ou de plusieurs particules, à l'oeil humain avec un grossissement inférieur à x 500 et sans amplification électronique. Le procédé comprend également la détection de la lumière diffusée par une de ces particules dans les conditions citées comme mesure de la présence des analytes.

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