C - Chemistry – Metallurgy – 12 – Q
Patent
C - Chemistry, Metallurgy
12
Q
195/1.1, 195/1.1
C12Q 1/68 (2006.01) G01N 33/58 (2006.01)
Patent
CA 1341365
Disclosed is a method of detecting in a sample an ana- lyte having a molecularly recognizable portion thereon. A detectable complex is formed comprising an analyte, a non- naturally-occurring molecular bridging entity comprising a portion capable of recognizing and complexing to the molecularly recognizable analyte portion and a portion comprising a poly- nucleotide sequence, and a signalling entity comprising a poly- nucleotide portion capable of annealing to and forming a stable polynucleotide hybrid with the bridging entity polynucleotide portion and a signal generating portion. The analyte is detected by a signal provided by the signal generating portion present in the detectable complex. Polynucleotide sequences and DNA mole- cules carrying a polynucleotide portion are provided. Also provided are a stable detectable complex and an entity capable of detecting a protein receptor.
546847
Engelhardt Dean L.
Kline Stan
Olsiewski Paula J.
Pergolizzi Robert G.
Rabbani Elazar
Cassan Maclean
Enzo Biochem Inc.
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