Analyte detection assay

G - Physics – 01 – N

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Details

G01N 33/533 (2006.01) G01N 33/546 (2006.01) G01N 33/551 (2006.01)

Patent

CA 2744331

A rapid and sensitive analyte detection assay is based on whispering gallery modes of fluorescently labelled microspheroidal particles. Ligands for the analyte, such as nucleic acids, are anchored to the particles. The fluorescent labels may comprise fluorophores or quantum dots. In the latter case, the particles may comprise melamine formaldehyde. The assay may be used to detect analytes in aqueous samples.

La présente invention concerne un essai de détection danalyte rapide et sensible basé sur des modes de galerie de particules microsphéroïdales marquées par fluorescence. Des ligands pour lanalyte, tels que des acides nucléiques, sont ancrés aux particules. Les marqueurs fluorescents peuvent comprendre des fluorophores ou des points quantiques. Dans le dernier cas, les particules peuvent comprendre du mélamine-formaldéhyde. Lessai peut être utilisé pour détecter des analytes dans des échantillons aqueux.

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