Analyte sensors and method for constructing analyte binding...

C - Chemistry – Metallurgy – 12 – Q

Patent

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C12Q 1/68 (2006.01) C12N 15/00 (2006.01) C12N 15/12 (2006.01) G01N 33/53 (2006.01) G01N 33/68 (2006.01) G01N 33/84 (2006.01) G06F 19/00 (2011.01)

Patent

CA 2576849

An analyte sensor with a tailored analyte binding motif that binds an analyte and a host protein in which the analyte binding motif is operatively linked or integrated therein and in which the binding of the analyte to the analyte binding motif produces a detectable change and manipulation of the analyte binding motif manipulates the responsiveness of the sensor. Also, a method for constructing an analyte sensor by constructing a tailored analyte binding motif capable of responding to an analyte and operatively inserting the analyte binding motif into a host protein. Included is a method for quantifying an analyte by introducing a nucleotide sequence for a protein having an analyte sensor with a tailored analyte binding motif that is able to produce a detectable change upon excitation, expressing the protein, providing excitement to the analyte sensor, and quantifying the detectable change.

L'invention concerne un capteur d'analyte à motif de liaison adapté qui se lie à un analyte et une protéine hôte, le motif de liaison d'analyte étant lié opérationnel ou intégré ; en outre, la liaison de l'analyte au motif produit une modification détectable et la manipulation du motif affecte la réactivité du capteur. On décrit aussi un procédé de construction de capteur d'analyte consistant à construire un motif de liaison d'analyte adapté capable de réagir à un analyte et à assurer l'insertion opérationnelle du motif dans une protéine hôte. On décrit aussi un procédé de quantification d'analyte par introduction de séquence nucléotidique pour une protéine à capteur d'analyte avec motif de liaison d'analyte adapté capable d'induire une modification détectable sur excitation, puis par expression de la protéine, excitation du capteur d'analyte, et quantification de la modification détectable.

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