Analytical compositions, elements and methods utilizing...

Details Analytical compositions, elements and methods utilizing... Analytical compositions, elements and methods utilizing...

C - Chemistry, Metallurgy

12

Q

150/14, 150/3

C12Q 1/26 (2006.01) G01N 33/52 (2006.01) G01N 33/94 (2006.01)

Patent

CA 1251383

-0- ANALYTICAL COMPOSITIONS, ELEMENTS AND METHODS UTILIZING REDUCTION OF FERRIC ION CHELATES TO FORM DETECTABLE DYES Abstract of the Disclosure Ferric ion chelates are useful in analytical compositions, elements and methods for the determina- tion of reductants, or other analytes which can produce a reductant. The ferric ion chelate is reduced to ferrous ion, which coordinates with a suitable ferrous ion coordinating ligand to form a colored complex. In particular, these compositions and elements are useful for detecting nicotinamide adenine dinucleotide, reduced form (NADH) or nicotin- amide adenine dinucleotide phosphate, reduced form (NADPH), or an analyte (e.g. an enzyme) which pro- duces NADH or NADPH in biological fluids.

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