Analytical element and method for alkaline phosphatase assay

Details Analytical element and method for alkaline phosphatase assay Analytical element and method for alkaline phosphatase assay

C - Chemistry, Metallurgy

12

Q

150/15.2

C12Q 1/42 (2006.01)

Patent

CA 1218290

-i- ANALYTICAL ELEMENT AND METHOD FOR ALKALINE PHOSPHATASE ASSAY Abstract of the Disclosure Disclosed herein is a dry analytical element and a method of using same for quantitatively detect- ing alkaline phosphatase in an aqueous liquid. The element comprises, in fluid contact, first and second reagent zones which can be self-supporting or carried on a support. The first reagent zone contains a substrate for alkaline phosphatase, e.g. p-nitro- phenyl phosphate, and the second reagent zone con- tains a buffer which is an alkali metal or ammonium salt and has a pKa in the range of 9-11.5, e.g. an alkali metal salt of carbonic acid.

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