Analytical element and method for determination of magnesium...

Details Analytical element and method for determination of magnesium... Analytical element and method for determination of magnesium...

G - Physics

01

N

150/14, 150/3

G01N 33/52 (2006.01) G01N 33/20 (2006.01) G01N 33/84 (2006.01)

Patent

CA 1290659

ANALYTICAL ELEMENT AND METHOD FOR DETERMINATION OF MAGNESIUM IONS Abstract of the Disclosure An analytical element is used to determine magnesium ions at a pH of from about 8.5 to about 11. This element comprises an absorbent carrier material containing a 1,5-bis(2-hydroxyphenyl)-3- cyanoformazan compound which is substituted in at least one of the 3-, 4- and 5-positions of either phenyl moiety. The substituents are chosen such that their cumulative Hammet-sigma value is greater than about 0.23, provided that none of the substituents is carboxy. The assay is carried out in the presence of a calcium ion chelating agent and a suitable buffer. In preferred embodiments, the cyanoformazan is incor- porated in the element in such a manner so as to be isolated from protein molecules which may be encoun- tered in the assay.

523820

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