G - Physics – 01 – N
Patent
G - Physics
01
N
G01N 33/558 (2006.01) G01N 33/543 (2006.01) G01N 33/58 (2006.01)
Patent
CA 2230802
An analytical element can be used to sensitively and rapidly detect a wide variety of specific binding ligands in either a competitive or sandwich assay format. The assays are carried out using a vanadium bromoperoxidase-labeled immunoreactant and a chemiluminescent signal-providing wash composition which comprises a 2,3-dihydro- 1,4- phthalazinedione derivative; a halogen, pseudohalogen, halogen-providing source or pseudohalogen-providing source; and a peroxide or a peroxide-generating reagent composition.
La présente invention concerne un élément analytique utilisable pour détecter avec sensibilité et rapidité une large gamme de ligands spécifiques de liaisons selon des techniques d'analyse dites "par compétition" ou "en sandwich". On effectue ces analyses en utilisant un immunoréactif étiqueté au bromoperoxydase de vanadium ainsi qu'une composition de lavage générant un signal de chimioluminescence, laquelle composition comprend un dérivé de 2,3-dihydro-1,4-phtalazinedione; un halogène, un pseudohalogène, une source apportant un halogène ou une source apportant un pseudohalogène; et un peroxyde ou une composition réactive générant un peroxyde.
Friedman Alan Eric
Groulx Sarah Fingar
Kissel Thomas Robert
Kopcienski Martha Miller
Gowling Lafleur Henderson Llp
Ortho-Clinical Diagnostics Inc.
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