Analytical element and method for theophylline determination...

Details Analytical element and method for theophylline determination... Analytical element and method for theophylline determination...

C - Chemistry, Metallurgy

12

Q

150/15.2, 150/3.

C12Q 1/42 (2006.01) G01N 33/52 (2006.01) G01N 33/94 (2006.01)

Patent

CA 1290662

IMPROVED ANALYTICAL ELEMENT AND METHOD FOR THEOPHYLLINE DETERMINATION HAVING INCREASED ALKALINE PHOSPHATASE ISOENZYME Abstract of the Disclosure Theophylline can be determined with an analytical element and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is carried out at a pH of 9 or less. The element comprises an absorbent carrier material, a suitable buffer and first and second zones in fluid contact. The isoenzyme of alkaline phosphatase and a suitable substrate for the isoenzyme are located in different zones of the element. The isoenzyme is present in one of the zones in an amount of at least about 100 I.U./m2. This level of isoenzyme reduces or eliminates bias caused by endogenous alkaline phosphatase and hemoglobin during an assay.

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