G - Physics – 01 – N
Patent
G - Physics
01
N
150/15.2, 150/3.
G01N 33/94 (2006.01) C12Q 1/00 (2006.01) C12Q 1/42 (2006.01)
Patent
CA 1251385
- 0 - ANALYTICAL ELEMENT, COMPOSITION, KIT AND METHOD FOR DETERMINATION OF THEOPHYLLINE BY ENZYME INHIBITION Abstract of the Disclosure Theophylline can be determined with an analytical element, composition, kit and method which utilize the inhibition, by theophylline, of alkaline phosphatase activity on an appropriate substrate. The assay is carried out at a pH of 9 or less. The element comprises an absorbent carrier material, a suitable buffer for maintaining the pH at 9 or less, and, in fluid contact, a first zone containing an isoenzyme of alkaline phosphatase which is capable of activity at pH 9 or less, and a second zone contain- ing a substrate for the isoenzyme. Use of this invention avoids the effect of endogenous alkaline phosphatase found in human biological fluids which has no activity at pH 9 or less.
480800
Eastman Kodak Company
Gowling Lafleur Henderson Llp
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