Analytical method and kit

Details Analytical method and kit Analytical method and kit

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01)

Patent

CA 2446310

Analytical methods using RNA probes for the detection or analysis of nucleic acid sequences is described. These probes are contacted with a sample suspected of containing the nucleic acid sequence and if they form duplexes, they are hydrolysed. This may be done, for example during an amplification reaction. AMP generated as a result of the hydrolysis is converted to ATP. The ATP may then be detected using bioluminescent reagents.

L'invention concerne des méthodes d'analyse mettant en oeuvre des sondes d'ARN aux fins de détection ou d'analyse de séquences d'acides nucléiques. Ces sondes sont mises en contact avec un échantillon soupçonné de renfermer la séquence d'acides nucléiques et s'ils forment des duplexes, ils sont soumis à une hydrolyse. Une telle opération peut être exécutée, par exemple, pendant une réaction d'amplification. L'adénosine monophosphate (AMP) obtenu par hydrolyse est converti en adénosine triphosphate (ATP) pouvant être ensuite détecté au moyen de réactifs bioluminescents.

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