Analytical process for the determination of a reduced coenzyme

Details Analytical process for the determination of a reduced coenzyme Analytical process for the determination of a reduced coenzyme

C - Chemistry, Metallurgy

12

Q

150/15.1

C12Q 1/28 (2006.01) C12Q 1/54 (2006.01) C12Q 1/58 (2006.01) G01N 33/52 (2006.01)

Patent

CA 1301037

A B S T R C T On met l'échantillon analytique contenant le coenzyme à déter- miner, notamment NADH ou NADPH, en présence d'un composé aromatique fluoré. En présence d'oxygène et de peroxydase, ce composé fluoré libère des ions fluorure à une vitesse proportionnelle à la quanti- té présente de coenzyme à déterminer. On dose les ions F- ainsi for- més ce qui conduit au résultat recherché.

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