Analytical rotors and methods for analysis of biological fluids

B - Operations – Transporting – 01 – D

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B01D 21/26 (2006.01) G01N 15/05 (2006.01) G01N 21/07 (2006.01) G01N 33/49 (2006.01) G01N 35/00 (2006.01)

Patent

CA 2082827

An analytical rotor (10), for sepa- rating cellular components from a bio- logical sample includes a plurality of in- ternal chambers and passages for com- bining the cell-free sample with one or more reagents and distributing the sam- ple to a plurality of individual test wells (92). The chambers and passages neces- sary for separating cellular components from a sample such as whole blood in- clude a measuring chamber (40), an overflow chamber (44), a separation chamber (60), and a reagent chamber (80), The measuring chamber (40) and overflow chamber (44) have capillary di- mensions. so that an initial volume of whole blood partitions therebetween, with the measuring chamber filling first to provide a preselected volume. The se- paration chamber (60) is located radially outward from both the measuring cham- ber (40) and the reagent chamber (80) so that spinning of the rotor causes both the reagent and the measured blood vo- lume to flow outward into the separation chamber (60). A collection chamber (90) is formed to receive the plasma from the separation chamber (60). Further spinning of rotor causes the plasma to flow radially outward into a plurality of test wells (92) formed around the periphery of the collec- tion chamber (90). Testing of the separated plasma volumes may be performed without removing the plasma from the rotor.

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