Angiogenic peptides

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 7/06 (2006.01) A61K 38/04 (2006.01) C07K 5/083 (2006.01) C07K 5/103 (2006.01) C07K 14/52 (2006.01) A61K 38/00 (2006.01)

Patent

CA 2098921

The present invention relates to peptides and peptide derivatives related to platelet factor 4 which exhibit angiogenic activity, to pharmaceutical compositions comprising said peptides, and to methods for promoting angiogenesis utilizing said peptides. It is based, in part, on the discovery that an octapeptide derived from platelet factor 4 and seven structurally related peptides (depitted in Figure 1) were capable of inducing an angiogenic response in vivo as measured by neovascularization in rabbit corneal implant assay and by measurement of capillary endothelial cell chemoattraction. These eight peptides represent specific nonlimiting, embodiments. of the present invention. The angiogenic peptides of the invention may be particularly useful in promoting wound healing, including incisional healing, bone repair, burn healing, and post-infarction repair in myocardial or central nervous system injury; and the assimilation of grafted tissues, particularly in persons suffering from vascular insufficiency, such as diabetic patients:

L'invention concerne des peptides et des dérivés de peptides relatifs au facteur plaquettaire 4 qui présentent une activité angiogénique, ainsi que des compositions pharmaceutiques comprenant ces peptides, et des procédés favorisant l'angiogénèse grâce à l'utilisation de ces peptides, l'invention se base, en partie, sur la découverte qu'un octapeptide dérivé du facteur plaquettaire 4 et sept peptides apparentés structurellement (Figure 1) se sont avérés capables d'induire une réponse angiogénique in vivo mesurée par néovascularisation dans une analyse d'un implant cornéen chez le lapin par mesure de la chimio-attraction des cellules endothéliales capillaires. Ces huit peptides représentent des modes de réalisation spécifiques non limitatifs de la présente invention. Les peptides angiogéniques de l'invention peuvent être particulièrement utiles pour la cicatrisation de blessures, y compris la cicatrisation d'incisions, la réparation osseuse, la cicatrisation de brûlures, et la réparation post-infarctus de blessures du système nerveux central ou du myocarde; et l'assimilation de tissus greffés en particulier chez des personnes souffrant d'insuffisances vasculaires, telles que les diabétiques.

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