Animal 2-5a-dependent rnases and encoding sequences therefor

Details Animal 2-5a-dependent rnases and encoding sequences therefor Animal 2-5a-dependent rnases and encoding sequences therefor

C - Chemistry, Metallurgy

12

N

C12N 15/55 (2006.01) C12N 9/22 (2006.01)

Patent

CA 2135307

2135307 9420605 PCTABS00033 Isolated 2-5A-dependent RNases, an interferon-induced enzyme which is activated by 5'-phosphorylated, 2',5'-linked oligoadenylates (2-5A) and implicated in both the molecular mechanisms of interferon action and in the fundamental control of RNA stability in mammalian cells (see Figs. 1 and 11), and encoding sequences therefor (see Figs. 3, 4, 9 and 10) are disclosed. The expression cloning and analysis of murine and human 2-5A-dependent RNases are also disclosed. Recombinant human 2-5A-dependent RNase produced in vitro and bound an activating affinity matrix, 2-5A-cellulose, have ribonuclease activity. The 2-5A binding properties of the recombinant and naturally occuring forms of 2-5A-dependent RNase are basically identical. Interferon induction of 2-5A-dependent RNase expression is demonstrated by measuring the mRNA levels in cells treated with interferon and cycloheximide. Analysis of aligned murine and human 2-5A-dependent RNase sequences revealed several features, including similarity to RNase E which is implicated in the control of mRNA stability in E. coli.

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