C - Chemistry – Metallurgy – 12 – N
Patent
C - Chemistry, Metallurgy
12
N
C12N 5/07 (2010.01) C12N 5/02 (2006.01) C12N 7/00 (2006.01) C12N 7/02 (2006.01)
Patent
CA 2517327
The present invention relates to a process for animal, preferably human, diploid anchorage-dependent cell culture, in the absence of exogenous components of primary animal origin, and to a cell culture medium substantially free of exogenous components of primary animal origin suitable for carrying out said process. In particular the invention concerns a cell culture medium which comprises at least one, more preferably several, exogenous animal-free growth factors. The present invention also relates to a process for cultivating animal, preferably human diploid anchorage-dependent cells in a medium according to the invention, involving the use of a trypsin substitute of non-animal origin for passaging cells. The invention further relates to a process for producing viruses, viral vaccines and the like.
L'invention porte sur un procédé de culture de cellules diploïdes (animales ou de préférence humaines) à dépendance d'ancrage, en l'absence de composants exogènes d'origine animale primaire, et sur un milieu de culture sensiblement exempt de composants exogènes d'origine animale primaire. L'invention porte également sur un milieu de culture de cellules comprenant au moins un, et de préférence plusieurs, facteurs de croissance exogènes non animaux, et sur un procédé de culture de cellules diploïdes (animales ou de préférence humaines) à dépendance d'ancrage, dans le milieu de l'invention, utilisant un substitut de la trypsine d'origine non animale pour assurer le passage des cellules. L'invention porte également sur un processus de production de virus, de vaccins antiviraux et autres.
Aerts Brigitte Ghislaine Louise
Ghislain Yves Jules Maurice
Gonze Marie-Monique Jane
Knott Isabelle Solange Lucie
Maggetto Carine
Glaxosmithkline Biologicals S.a.
Norton Rose Or S.e.n.c.r.l. S.r.l./llp
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