Annealing control primer and its uses

C - Chemistry – Metallurgy – 12 – P

Patent

Rate now

  [ 0.00 ] – not rated yet Voters 0   Comments 0

Details

C12P 19/34 (2006.01) C07H 21/00 (2006.01) C07H 21/04 (2006.01) C12N 15/09 (2006.01) C12Q 1/68 (2006.01)

Patent

CA 2468754

The present invention relates to an annealing control primer for improving annealing specificity in nucleic acid amplification and its applications to all fields of nucleic acid amplification-involved technology. The present primer comprises (a) a 3'-end portion having a hybridizing nucleotide sequence substantially complementary to a site on a template nucleic acid to hybridize therewith; (b) a 5'-end portion having a pre-selected arbitrary nucleotide sequence; and (c) a regulator portion positioned between said 3'-end portion and said 5'-end portion comprising at least one universal base or non- discriminatory base analog, whereby said regulator portion is capable of regulating an annealing portion of said primer in association with annealing temperature.

L'invention concerne une amorce de commande de renaturation servant à améliorer la spécificité de renaturation dans l'amplification d'acides nucléiques, ainsi que ses applications à tous les domaines de technologie impliquant l'amplification d'acides nucléiques. L'amorce selon l'invention comprend : (a) une partie d'extrémité 3' présentant une séquence nucléotidique d'hybridation pratiquement complémentaire d'un site sur un acide nucléique matrice pour l'hybridation ; (b) une partie d'extrémité 5' présentant une séquence nucléotidique arbitraire présélectionnée et (c) une partie régulatrice située entre la partie d'extrémité 3' et la partie d'extrémité 5' et comprenant au moins un analogue de base universelle ou de base non discriminatoire. Selon l'invention, cette partie régulatrice est apte à réguler une partie de renaturation de l'amorce, en association avec la température de renaturation.

LandOfFree

Say what you really think

Search LandOfFree.com for Canadian inventors and patents. Rate them and share your experience with other people.

Rating

Annealing control primer and its uses does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Annealing control primer and its uses, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Annealing control primer and its uses will most certainly appreciate the feedback.

Rate now

     

Profile ID: LFCA-PAI-O-1824519

  Search
All data on this website is collected from public sources. Our data reflects the most accurate information available at the time of publication.