Anti-interferon alpha monoclonal antibodies and methods for use

C - Chemistry – Metallurgy – 12 – N

Patent

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C12N 15/13 (2006.01) A61K 39/395 (2006.01) A61P 37/02 (2006.01) C07K 16/24 (2006.01) C12N 5/16 (2006.01) C12P 21/08 (2006.01)

Patent

CA 2597265

The present invention includes compositions and methods that include antibodies that selectively neutralize a bioactivity of at least two interferon alpha ("IFN.alpha.") protein subtypes for the protein subtypes A, 2, B2, C, F, G, H2, 1, Jl, K, 4a, 4b and WA, but does not neutralize at least one bioactivity of IFN.alpha. protein subtype D. Examples of bioactivity for measurement include activation of the MxA promoter or antiviral activity and variants, derivatives and fragments thereof. The invention also includes host cells, hybridomas and plasmacytomas that produce antibodies. Because of their unique selectivity and affinity, the antibodies of the present invention are useful to detect IFN.alpha. subtypes in sample or tissue and/or for therapeutic applications that include, but are not limited to the treatment and/or amelioration of an IFN.alpha. related disorder such as SLE, lupus, type I diabetes, psoriasis, AIDS and Graft versus Host Disease.

La présente invention concerne des compositions et des procédés qui comprennent des anticorps qui neutralisent sélectivement une bioactivité d'au moins deux sous-types de protéine d'interféron alpha ("IFN.alpha.") pour les sous-types de protéine A, 2, B2, C, F, G, H2, I, J1, K, 4a, 4b et WA, mais qui ne neutralisent pas au moins une bioactivité du sous-type D de protéine IFN.alpha.. Des exemples de bioactivité à mesurer comprennent l'activation du promoteur MxA ou l'activité antivirale et des variantes, dérivés et fragments associés. Cette invention concerne également des cellules hôtes, des hybridomes et des plasmacytomes qui produisent des anticorps. En raison de leur sélectivité et de leur affinité uniques, les anticorps de cette invention sont utilisés pour détecter des sous-types d'IFN.alpha. dans un échantillon ou un tissu et/ou dans des applications thérapeutiques parmi lesquelles figurent notamment le traitement et/ou l'amélioration d'un trouble associé à l'INF.alpha., tel que le lupus érythémateux disséminé (LED), le lupus, le diabète de type 1, le psoriasis, le SIDA, et la maladie du greffon contre l'hôte.

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