Anti-neoplastic viral agents

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 35/76 (2006.01) C07K 14/075 (2006.01) C12N 7/00 (2006.01) C12N 15/34 (2006.01) C12N 15/861 (2006.01)

Patent

CA 2453357

A viral DNA construct, and virus encoded thereby, is provided having one or more tumour specific transcription factor binding sites in place of one or more wild type transcription factor binding sites operatively positioned in the promoter region which controls expression of E1A open reading frame. Preferred constructs place the tumour specific transcription factor binding sites in operative relation to DNA polymerase, DNA terminal protein and/or DNA binding protein. Compositions and constructs contained therein are provided, particularly for use in therapy. Methods of treating patients for neoplasms are also provided.

L'invention concerne une construction d'ADN virale et un virus codé par cette construction, qui présente au moins un site de liaison du facteur de transcription spécifique de tumeurs au lieu d'au moins un site de liaison de facteur de transcription de type sauvage, placé fonctionnellement dans la région promoteur qui commande l'expression de la phase de lecture ouverte (E1A). Des constructions préférées mettent les sites de liaison du facteur de transcription spécifique de tumeurs en liaison fonctionnelle avec l'ADN polymérase, la protéine terminale d'ADN et/ou la protéine de liaison d'ADN. L'invention concerne également des compositions et des constructions contenues dans celle-ci, destinées en particulier à être utilisées en thérapie, ainsi que des procédés de traitement de patients présentant des néoplasmes.

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