Anti-sars monoclonal antibodies

Details Anti-sars monoclonal antibodies Anti-sars monoclonal antibodies

C - Chemistry, Metallurgy

12

N

C12N 15/13 (2006.01) A61K 39/125 (2006.01) A61K 39/395 (2006.01) C07K 16/10 (2006.01) C07K 16/46 (2006.01) C12P 21/08 (2006.01) G01N 33/569 (2006.01)

Patent

CA 2548942

Monoclonal antibody reagents that recognize the SARS-coronavirus (SARS-HCoV) are needed urgently. In this report we describe the development and immunochemical characterisation of mAbs against the SARS-HCoV based upon their specificity, binding requirements, and biological activity. Initial screening by ELISA, using highly purified virus as the coating antigen, resulted in the selection of seventeen mAbs. Five mAbs exhibited Western immunoblot reactivity with the denatured spike protein, of which two demonstrated the ability to neutralize SARS-HCoV in vitro. Another four Western immunoblot-negative mAbs also neutralize the virus. These antibodies will be useful for the development of diagnostic tests, pathogenicity and vaccine studies.

L'invention concerne des réactifs d'anticorps monoclonaux qui reconnaissent le coronavirus SARS (SARS-HCoV) impérativement nécessaires. Dans cette invention, on décrit le développement et la caractérisation immunochimique des mAbs contre le SARS-HCoV basé sur leur spécificité, les besoins de liaison et l'activité biologique. Un criblage initial par dosage immunoenzymatique (ELISA), utilisant un virus hautement purifié comme antigène de revêtement, entraîne une sélection de dix-sept mAbs. Cinq mAbs présentent une réactivité immunoblot de type Western avec la protéine de spicule dénaturée, dont deux ont démontrés la capacité à neutraliser le SARS-HCoV in vitro. Quatre autres mAbs immunoblot négatifs de type Western neutralisent également le virus. Ces anticorps sont utiles dans le développement de tests de diagnostic, de recherches de pathogénicité et de vaccin.

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