Anti-tenascin monoclonal antibody immunoassays and...

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 1/00 (2006.01) C07K 16/00 (2006.01) G01N 33/53 (2006.01)

Patent

CA 2587638

The present invention provides immunoassays for detecting a tumor in a subject, comprising producing an antibody that specifically binds to tenascin, contacting the antibody with a biological sample suspected of containing tumor cells and determining the binding of the antibody to the biological sample. The present invention further provides methods of identifying a subject for treatment of a tumor. Kits for direct or indirect immunohitochemical or immunocytochemical assays are also provided. A novel polyclonal antibody that binds to tenascin domain TNfn C-D is further provided.

L'invention concerne des dosages immunologiques permettant de détecter une tumeur chez un sujet. Ces dosages consistent à produire un anticorps qui se lie spécifiquement à la tenascine, à mettre cet anticorps en contact avec un échantillon biologique suspecté de contenir des cellules tumorales, et à déterminer la liaison de l'anticorps à l'échantillon biologique. L'invention concerne également des méthodes permettant d'identifier un sujet pour le traitement d'une tumeur. L'invention concerne encore des kits pour des dosages immunohistochimiques ou immunocytochimiques directs ou indirects. L'invention concerne enfin un nouvel anticorps polyclonal qui se lie au domaine tenascine TNfn C-D.

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