Anti-thymosin .alpha.1 monoclonal antibody-producing hybridoma

Details Anti-thymosin .alpha.1 monoclonal antibody-producing hybridoma Anti-thymosin .alpha.1 monoclonal antibody-producing hybridoma

C - Chemistry, Metallurgy

12

N

C12N 5/18 (2006.01) C07K 16/26 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01) G01N 33/74 (2006.01)

Patent

CA 2145905

There are disclosed a hybridoma obtained by fusing a myeloma cell with an anti-thymosin .alpha.1 antibody-producing cell and selecting a hybridoma which produces a monoclonal antibody that recognizes the N-terminus of thymosin .alpha.1; an anti- thymosin .alpha.1 monoclonal antibody which is produced by the hybridoma, and a method for measuring thymosin .alpha.1 which comprises forming a three-component complex comprising thymosin .alpha.1, the anti-thymosin .alpha.1 monoclonal antibody (A), and an anti-thymosin .alpha.1 antibody (B) that recognizes a different site of thymosin .alpha.1 than antibody (A) does; forming a four- component complex of said three- component complex with a labeled substance (C) that specifically binds to antibody (A) or antibody (B) of the three-component complex; and detecting the labeled substance in said four-component complex.

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