Anti-viral vectors

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) C12N 9/00 (2006.01) C12N 15/11 (2006.01) C12N 15/63 (2006.01) C12N 15/867 (2006.01) A61K 38/00 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2321097

A viral vector production system is provided which system comprises: (i) a viral genome comprising at least one first nucleotide sequence encoding a gene product capable of binding to and effecting the cleavage, directly or indirectly, of a second nucleotide sequence, or transcription product thereof, encoding a viral polypeptide required for the assembly of viral particles; (ii) a third nucleotide sequence encoding said viral polypeptide required for the assembly of the viral genome into viral particles, which third nucleotide sequence has a different nucleotide sequence to the second nucleotide sequence such that said third nucleotide sequence, or transcription product thereof, is resistant to cleavage directed by said gene product. The viral vector production system may be used to produce viral particles for use in treating or preventing viral infection.

L'invention porte sur un système de production de vecteurs antiviraux comprenant: (i) un génome viral comprenant au moins une séquence nucléotidique codant pour un produit génique pouvant se fixer à une deuxième séquence nucléotidique ou à ses produits de transcription et en effectuer directement ou indirectement le clivage, et codant pour le polypeptide viral requis pour l'assemblage des particules virales; (ii) une troisième séquence nucléotidique codant pour ledit polypeptide viral requis pour l'assemblage de génomes viraux en particules virales. Ladite troisième séquence, dont la séquence nucléotidique ou ses produits de transcription différent de ceux de la deuxième, résiste de ce fait au clivage sous l'effet dudit produit génique. Le susdit système peut servir à produire des particules virales servant au traitement ou à la prévention des infections virales.

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