Antibacterial therapy with genotypically modified bacteriophage

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 7/01 (2006.01) A61K 35/76 (2006.01) C07K 14/47 (2006.01) C12N 7/00 (2006.01) C12N 7/08 (2006.01) C12N 15/01 (2006.01)

Patent

CA 2186962

The present invention is directed to bacteriophage therapy, using methods that enable the bacteriophage to delay inactivation by any and all parts of the host defense system (HDS) against foreign objects that would tend to reduce the numbers of bacteriophage and/or the efficiency of those phage at killing the host bacteria in an infection. Disclosed is a method of producing bacteriophage modified for anti-HDS purposes, one method being selection by serial passaging, and the other method being genetic engineering of a bacteriophage, so that the modified bacteriophage will remain active in the body for longer periods of time than the wild-type phage.

Thérapie à l'aide de bactériophages dans laquelle on a recours à des procédés qui permettent aux bactériophages de retarder leur inactivation par une partie ou toutes les parties du système de défense endogène (HDS) contre des corps étrangers, système qui tendrait à réduire le nombre de bactériophages et/ou la propension des bactériophages à tuer les bactéries hôtes présentes dans une infection. Est décrit un procédé de production de bactériophages modifiés à des fins anti-HDS, consistant soit à sélectionner des bactériophages par administrations successives des bactériophages à un animal, soit à produire des bactériophages par génie génétique, de manière à ce que les bactériophages modifiés restent actifs à l'intérieur du corps plus longtemps que les bactériophages de type sauvage.

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