Antibiotic-free plasmid

Details Antibiotic-free plasmid Antibiotic-free plasmid

C - Chemistry, Metallurgy

12

N

C12N 15/73 (2006.01) C12N 15/63 (2006.01) C12N 15/68 (2006.01)

Patent

CA 2761964

The present invention provides a method of maintaining a gram negative bacterium plasmid without the use of antibiotic selection pressure. Further, the invention relates to the drugless plasmids produced including drugless plasmids containing a heterologous gene. The invention also provides formulations and/or compositions comprising the drugless plasmids comprising a heterologous gene, formulations and/or compositions comprising a protein or an immunogen expressed using the drugless plasmids, and methods of administering such formulations and/or compositions to a host. The invention relates to gram negative bacteria containing the drugless plasmids.

La présente invention concerne un procédé de stabilisation d'un plasmide de bactérie à Gram négatif qui ne fait pas appel à la pression de sélection des antibiotiques. De plus, l'invention concerne les plasmides exempts de médicament produits, notamment les plasmides exempts de médicament qui contiennent un gène hétérologue. L'invention concerne également des formulations et/ou compositions comprenant les plasmides exempts de médicament comprenant un gène hétérologue, des formulations et/ou compositions comprenant une protéine ou un immunogène exprimé à l'aide des plasmides exempts de médicament, et des méthodes d'administration de ces formulations et/ou compositions à un hôte. L'invention concerne des bactéries à Gram négatif contenant les plasmides exempts de médicament.

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