Antibiotic susceptibility testing

C - Chemistry – Metallurgy – 12 – Q

Patent

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73/124, 73/59

C12Q 1/00 (2006.01) C12M 1/34 (2006.01) G01N 21/25 (2006.01) G06F 19/00 (2006.01)

Patent

CA 1123629

ABSTRACT OF THE DISCLOSURE Method and apparatus are disclosed for detecting antibiotic sus- ceptibilities using serial dilution in liquid media. A plurality of dif- ferent antibiotics are placed in a large number of small wells in a trans- lucent plastic tray, each antibiotic being placed in a column of wells at serially diluted concentrations. A bacteria culture of known uniform con- centration is then inoculated into each well. Following a period sufficient to allow growth, a diffuse uniform light is passed through each of the wells and their contents, and the intensity of the light passing through each well is measured to determine turbidity of the bacterial suspension. A turbidity value for each well is compared with a value corresponding to zero growth, and this information is processed by a computer which calculates which of the antibiotics are effective to inhibit growth of the bacteria, and the minimum concentration of each effective antibiotic necessary to inhibit growth. This information is displayed and printed out and provides inter- pretive information to determine the choice and dosage of an antibiotic to be administered to the patient. The computer is programmed to perform var- ious calibration operations and checking routines to confirm that the in- strument is operating properly and that the test is being conducted properly.

315138

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