Antibiotics based upon bacteriophage lysis proteins

C - Chemistry – Metallurgy – 12 – Q

Patent

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Details

C12Q 1/68 (2006.01) C07K 14/00 (2006.01) C07K 14/01 (2006.01) C07K 14/08 (2006.01)

Patent

CA 2390371

This invention relates to polypeptide antibiotics, including materials and methods related thereto, based upon the observation that bacteriophage elaborate proteins that cause host cell lysis by interfering with specific steps in cell wall biosynthesis. Examples of antibiotics based upon this invention include the bacteriophage ~X174 gene E product and structurally and/or functionally related polypeptide and small protein antibiotics that interact with MraY, and the bacteriophage Q.beta. gene A2 product and structurally and/or functionally related polypeptide and small protein antibiotics that interact with MurA. This leads to the general model for obtaining new polypeptide antibiotics by using genetic approaches based on these findings to find polypeptide sequences which cause bacterial cell lysis.

La présente invention concerne des antibiotiques polypeptidiques, qui comprennent des matériaux et des procédés y afférents. Cette invention se fonde sur l'observation que des protéines bactériophages complexes induisent la lyse de cellules hôtes par une interférence dans des étapes spécifiques de la biosynthèse des membranes cellulaires. Des exemples d'antibiotiques fondés sur cette invention comprennent le produit génique E ~X174, le polypeptide structurellement et/ou fonctionnellement lié et les antibiotiques à petites protéines qui interagissent avec MraY, et le produit génique A¿2? Q.beta. bactériophage, le polypeptide structurellement et/ou fonctionnellement lié et les antibiotiques à petites protéines qui interagissent avec MurA. Ces exemples débouchent sur le modèle général permettant d'obtenir de nouveaux antibiotiques polypeptidiques par des techniques génétiques fondés sur ces découvertes de façon à trouver des séquences de polypeptides qui induisent la lyse de cellules bactériennes.

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