Antibodies against amyloid beta 4 with glycosylated in the...

C - Chemistry – Metallurgy – 07 – K

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Details

C07K 16/18 (2006.01) A61K 39/395 (2006.01) A61P 25/28 (2006.01)

Patent

CA 2632828

The present invention relates to a purified antibody molecule preparation being characterized in that at least one antigen binding site comprises a glycosylated asparagine (Asn) in the variable region of the heavy chain (VH). More specifically, a pharmaceutical and a diagnostic composition comprising said antibody molecule and antibody mixtures are provided which is/are capable of specifically recognizing the .beta.-A4 peptide/A.beta.4. The present invention relates in particular to a mixture of antibodies comprising one or two glycosylated antigen binding sites with a glycosylated asparagine (Asn) in the variable region of the heavy chain, i.e. mixtures of isoforms of antibodies which comprise a glycosylated Asn in the variable region of the heavy chain (VH). Also disclosed are compositions or antibody preparations comprising the specifically glycosylated antibody isoforms. Furthermore, the pharmaceutical and diagnostic uses for these antibodies are provided. The antibody isoforms may for example be used in the pharmaceutical intervention of amyloidogenesis or amyloid-plaque formation and/or in the diagnosis of the same.

La présente invention concerne une préparation de molécules d~anticorps purifiées caractérisée en ce qu'au moins un site se liant à l'antigène comprend une asparagine (Asn) glycosylée dans la région variable de la chaîne lourde (VH). Plus spécifiquement, la présente invention concerne un produit pharmaceutique et une préparation pour diagnostic comprenant ladite molécule d'anticorps et des mélanges d'anticorps capables de reconnaître de façon spécifique le peptide .beta.-A4/A.beta.4. La présente invention concerne en particulier un mélange d'anticorps comprenant un ou deux sites se liant à l'antigène glycosylés comprenant une asparagine (Asn) glycosylée dans la région variable de la chaîne lourde, c'est-à-dire des mélanges d'isoformes d'anticorps comprenant une Asn glycosylée dans la région variable de la chaîne lourde (VH). La présente invention concerne également des formules ou des préparations d'anticorps comprenant les isoformes d'anticorps spécifiquement glycosylées. En outre, la présente invention concerne les utilisations pharmaceutiques et diagnostiques de ces anticorps. Les isoformes d'anticorps peuvent par exemple être employées dans le traitement pharmaceutique de l~amyloïdogénèse ou la formation de plaques amyloïdes et/ou dans le diagnostic desdites affections.

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