C - Chemistry – Metallurgy – 07 – K
Patent
C - Chemistry, Metallurgy
07
K
C07K 16/18 (2006.01) C12N 5/12 (2006.01) G01N 33/569 (2006.01) G01N 33/68 (2006.01)
Patent
CA 2408762
Antibodies for specifically detecting pathogenic prions of human origin, and methods for detecting pathogenic prions, are described. In particular, a conformation- dependent immunoassay method for detecting pathogenic prion proteins in a sample of a body fluid, containing a PrP protein, which contains a first, natural, non- pathological conformation, i.e. PrP c, and a second, pathological conformation, i.e. PrP sc, is described, in which method the prion proteins differ in their binding affinity for monoclonal antibodies which bind specifically to prion proteins of human origin, with the detection method comprising the following steps: a) adding one of the abovementioned monoclonal antibodies, which is fixed to a solid support and which exhibits a higher affinity for the first prion protein conformation, to the first portion of the sample, and determining this first concentration; b) treating the second portion of the sample in order to increase the binding affinity of the second conformation of the prion protein for the monoclonal antibody; c) adding the monoclonal antibody to the treated second portion of the sample to be investigated, in order to determine the second concentration; d) comparing the first prion protein concentration with the second prion protein concentration in order to ascertain the presence of the pathogenic prion protein conformation.
Bellon Anne
Groener Albrecht
Lang Wiegand
Vey Martin
Aventis Behring Gmbh
Bereskin & Parr Llp/s.e.n.c.r.l.,s.r.l.
Csl Behring Gmbh
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