Antibodies to human leucocyte interferon

C - Chemistry – Metallurgy – 12 – N

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C12N 15/00 (2006.01) C07K 14/555 (2006.01) C07K 16/24 (2006.01) G01N 33/577 (2006.01) G01N 33/68 (2006.01)

Patent

CA 1213226

Abstract There is described a collection of monoclonal anti- bodies the members of which being directed against human leucocyte interferon (HLI) which is natural or obtained by recombinant DNA technology. The mono- clonal antibodies are preferably secreted by different hybridomas. It has been shown that individual mono- clonal antibodies are not mutually inhibiting, i.e. bind together to HLI while others are mutually in- hibiting, i.e. do not bind together to HLI. It has been shown furthermore that the collection consists of groups of antibodies/members of which recognize different epitopes (antigenic determinants) of HLI. Each a member of the different groups of antibodies can be used for a solid phase-sandwich test for HLI. In addition an antibody of the collection can be used for the purification of HLI by affinity chromatography.

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