Antibodies to ligand analogues and their utility in...

Details Antibodies to ligand analogues and their utility in... Antibodies to ligand analogues and their utility in...

C - Chemistry, Metallurgy

12

Q

C12Q 1/68 (2006.01) A61K 39/395 (2006.01) G01N 33/53 (2006.01) G01N 33/541 (2006.01) G01N 33/543 (2006.01) G01N 33/566 (2006.01) G01N 33/577 (2006.01)

Patent

CA 2071904

Means for the detection of free ligand analogue conjugate in fluids from competitive ligand-receptor assay processes. Li- gand analogue antibodies that bind the ligand analogue conjugate with substantially greater affinity than their affinity for target ligand are selected and used in competitive ligand-receptor assay processes to bind the free fraction of the ligand analogue conju- gate. This means permits the detection of the free fraction of ligand analogue conjugate even in the presence of substantially high- er concentrations of free target ligand. For the purposes of the present invention, ligand analogue antibodies are antibodies that exhibit at least 100 X greater affinity for the ligand analogue conjugate compared to their affinity for the target ligand.

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