Antibody binding affinity ligands

C - Chemistry – Metallurgy – 07 – K

Patent

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Details

C07K 5/10 (2006.01) C07K 5/08 (2006.01) G01N 33/543 (2006.01)

Patent

CA 2591785

The present application discloses a solid support material having covalently immobilized thereon an affinity ligand, said ligand comprising one or more hydrophobic functional group(s) and one or more cationic functional group(s) or one or more heteroaromatic functional group(s), wherein at least one hydrophobic functional group is separated from at least one cationic/heteroaromatic functional group by a through bond distance of from 5 ~ to 20 ~, wherein said ligand has a molecular weight of from 120 Da to 5,000 Da. Typically, the affinity resin has a binding capacity larger than 5 mg monoclonal antibody per mL of affinity resin. A method for the isolation of biomolecules, such as proteins, in particular antibodies, such as monoclonal antibodies, or derivatives thereof, is also disclosed.

La présente invention concerne un matériau support solide sur lequel est immobilisé un ligand affinitaire comprenant au moins un groupe fonctionnel hydrophobe, et au moins un groupe fonctionnel cationique ou un ou plusieurs groupes fonctionnels hétéroaromatiques. En l'occurrence, le groupe fonctionnel hydrophobe est séparé d'au moins un groupe fonctionnel cationique ou hétéroaromatique par une distance de liaison de 5 Å à 20 Å, la masse moléculaire du ligand allant de 120 Da à 5.000 Da. D'une façon générale, la résine affinitaire présente une capacité de liaison supérieure à 5 mg d'anticorps monoclonal par ml de résine affinitaire. L'invention concerne également un procédé d'isolation de biomolécules telles que des protéines, et plus particulièrement d'anticorps tels que des anticorps monoclonaux, ou certains de leurs dérivés.

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