Antibody dependent enhancement (ade) of alphavirus infection

C - Chemistry – Metallurgy – 12 – N

Patent

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Details

C12N 15/86 (2006.01) A61K 39/00 (2006.01) C12N 15/44 (2006.01) C12N 15/87 (2006.01) A61K 48/00 (2006.01)

Patent

CA 2383519

The present invention provides compositions and methods for delivering a nucleotide sequence to a cell using an alphavirus vector that is complexed with an enhancing antibody that specifically binds to the alphavirus vector. Venezuelan Equine Encephalitis vectors are preferred. The cell may be a cell in vitro or in vivo. Alternatively, the cell may be removed from a subject, administered the alphavirus vector ex vivo and then administered to a subject. Antigen-presenting cells are preferred, with dendritic cells being more preferred. Also provided are methods of producing an immune response in a subject, e.g., for producing an immune response against an antigen associated with a pathogen or for immunotherapy of cancer of tumors.

L'invention concerne des compositions et des procédés permettant de fournir une séquence nucléotidique à une cellule au moyen d'un vecteur d'alphavirus complexé avec un anticorps facilitant qui se lie spécifiquement au vecteur d'alphavirus. Des vecteurs du virus de l'encéphalite équine du Venezuela sont préférés. On peut éventuellement retirer la cellule d'un sujet, introduire le vecteur d'alphavirus dans cette cellule ex-vivo puis réintroduire la cellule dans le sujet. On utilise de préférence des cellules présentant des antigènes, et idéalement des cellules dendritiques. L'invention concerne également des procédés de production d'une réponse immunologique chez un sujet, p. ex. contre un antigène associé à un pathogène ou pour une immunothérapie contre le cancer.

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